4-(2-Hydroxyethyl)-1-Piperazineethanesulfonic Acid,7365-45-9

タイトルまたは説明

HEPES, *-(*-Hydroxyethyl)**-Piperazineethanesulfonic Acid, is a kind of zwitterionic buffer, which is often used in biochemical experiments to regulate the pH value in the reaction system. For example, adding HEPES to virus preservation solution or cell preservation solution is non-toxic to cells and can maintain a constant pH range for a long time.

HEPES powder is resistant to high temperature and its melting point is up to **0 ℃. However, HEPES aqueous solution will produce toxic hydrogen peroxide in the light environment. Therefore, the use and preservation of HEPES aqueous solution should pay attention to shading and avoiding light.

HEPES is widely used, mainly in the following aspects:
1. For cell culture: the decomposition ability of HEPES is less affected by the temperature change, which makes HEPES maintain the structure and function of enzyme at low temperature.

2. For the study of metal ions: HEPES buffer is often used in the study of various metal ions. Based on the characteristics of the buffer, HEPES can better replace Tris and phosphate buffer in the study of metal ions.

3. For skin care products: HEPES is harmless to human body, safe and stable, and its pH maintaining property can help skin care products maintain the activity of microbial fermentation extract and prolong the efficacy; HEPES can promote the absorption of various components in skin care products, and improve the skin's absorption rate of cosmetics; HEPES can also soften cutin, enhance cell metabolism, and cooperate with sunscreen.

4. For virus preservation solution: HEPES can be used for non inactivated virus preservation solution. The maintenance of solution pH by HEPES can increase the survival time of virus host cells in vitro and maintain the integrity of virus nucleic acid and protein to the greatest extent.

5. For the preparation of gold nanoparticles: hepes-naoh reduction method is used to prepare gold nanoparticles based on the reduction of excessive metals by HEPES at a specific pH value. This method is simple and rapid.

6. For cell preservation medium: cell culture medium generally relies on sodium bicarbonate and CO2 for buffering, but CO2 is unstable in the solution, easy to escape, resulting in increased pH. In this case, the addition of HEPES can improve the above problems and maintain a stable pH value of the culture medium.

Our company can produce HEPES buffer products with excellent quality and reasonable price. For more information, please contact us: Hubei xindesheng Material Technology Co., Ltd