タイトルまたは説明
Carboxypeptidase B (CPB)
Carboxypeptidase B (CPB) (EC3.4.*7.2) is an exocrine protease,
belonging to the family of metallocarboxypeptidase A and
carboxypeptidase B (CPA / CPB). Each molecule contains a Zn atom,
which can selectively hydrolyze arginine and lysine at the carboxyl
end of protein or peptide. Carboxypeptidase B preferentially
cleaves basic amino acids, but CPB can also cleave some non basic
amino acids. The molecular weight of CPB is *5kD, the isoelectric
point is 6.0, and the optimal pH is **9. The activity of CPB is
competitively inhibited by lysine or arginine, and the activity of
CPB is inhibited by metal ion chelating agents such as EDTA. The
application of carboxypeptidase B in scientific research is mainly
based on its cutting characteristics. It can be used to sequence
proteins and peptides and to diagnose pancreatitis in medicine. In
addition, in the production of recombinant insulin, the production
of active insulin also depends on the cutting of carboxypeptidase
B.
CPB of our company is a recombinant product,with high purity, good
activity, free of animal origin virus, such as swine influenza
virus and other pollution.
[Product Description]
Dosage form: freeze dried powder
Preservation buffer: *0mM Tris, pH 8.0
Enzyme activity: **0 U / mg protein
Activity unit: under the condition of *5 ℃, pH 7.*5, the
enzyme quantity of 1 um Hip-L-Arg hydrolysis per minute is defined
as 1 u unit.
General information:
Source: gene recombinant expression
Molecular weight: about *5 kDa (SDS-PAGE detection)
Purity: ≥ *0% (SDS-PAGE detection)
Storage conditions: freeze dried powder at - *0 ℃ or long-term
storage at 4 ℃
Transportation conditions: ice bag at low temperature
Safety tips: not for human experiment
