Globetech Systems(Asia) Limited

Shenzhen,Guangdong,China

無料会員

CENTRI-SEP Spin Columns

China

CS-900, CS-901

( Negotiable ) Get Latest Price

USA

-

1 Piece

-

3-4 weeks

100 Piece per Month

T/T

-

製品仕様

Princeton

-

タイトルまたは説明

The column design is based on the description of gel filtration for the purification of DNA from nick translation reactions by Sambrook, et.al. Each Centri-Sep unit consists of a specially fritted microfuge tube, dry gel, a wash tube and a sample collection tube, all specifically designed for this purpose.

The Centri-Sep gel will provide excellent recovery of DNA fragments with sizes greater than 16 base pairs or 25-mer, while removing > 98 % of salts, NTP's and other unwanted low- molecular- weight impurities.

The column gel is hydrated with either reagent-grade water or a suitable buffer or your choice, and spun in a microcentrifuge or swinging-bucket centrifuge to remove the intersititial fluid. Your sample is then applied to the column and spun again, leaving you not only with a sample purified through removal of low- molecular- weight components, but also exchanged into the buffer of your choice.

 

Centri-Sep Columns can also be used for the following applications:
  • Removal of excess DyeDeoxy™ terminators
  • Removal of excess BigDye™ terminators
  • Removal of more than 98% of salts and unwanted low-molecular-weight impurities
  • Purification of fluorescent reaction mixtures
  • Removal of free and labeled dNTP's from DNA or RNA, as inon:
  1. nick translation
  2. end-labeling reactions
  3. polymerization reaction
  • Removal of traces of phenol or exchange of buffer salts
  • Purification and desalting of proteins
  • Removal of excess nucleotides and small primers in PCR reactions
  • Removal of radioactive precursors
  • Desalting and buffer exchange
  • These columns are far superior – easy to use, quick protocol, non-toxicity – than phenol/chloroform extraction or ethanol precipitation.
Advantages of Centri-Sep spin column:
  • Processed faster than ultrafiltration columns
  • Rapid and efficient separations
  • Buffer is not preselected
  • Convenient 20-100 µL sample size
  • Dry gel format
  • Room Temperature Storage
  • Microcentrifuge format
Benefits of Centri-Sep spin column:
  • Reliable and consistent
  • Time-saving and convenient
  • Enhanced stability
  • Extended shelf-life
  • No special equipment
  • Extremely versatile.
Price and ordering information: 32 Column Kit - Product No: CS-900 
100 Column Kit - Product No: CS-901 

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Globetech Systems(Asia) Limited

連絡先担当者

Andrea

会社

Globetech Systems(Asia) Limited

住所

Shenzhen, Guangdong, China,

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