タイトルまたは説明

This manual provides sample-specific protocols to isolate total RNA from a wide range of sample types and amounts.In general, samples are lysed and then homogenized in the presence of guanidinium isothiocyanate, a chaotropic salt capable of protecting the RNA from endogenous RNases. After homogenization, ethanol is added to the sample. The sample is then processed through a Spin Cartridge containing a clear silica-based membrane to which the RNA binds. Any impurities are effectively removed by subsequent washing. The purified total RNA is then eluted in RNase-Free Water  and is suitable for use in a variety of downstream applications.