HIV 1+2 Antibody ELISA Kit

タイトルまたは説明

 Human immunodeficiency viruses (HIV) are retroviruses transmitted via sexual contact, parenteral and perinatal pathways or the placenta. HIV*1 and HIV*2 were respectively isolated  in  ***3  and  ***5  in  patients  with  AIDS  (Acquired  Immunodeficiency Syndrome). Serological evidence of HIV infection may be obtained by testing for HIV antigens or antibodies in serum of individuals suspected of HIV infection. Antigens can generally be  detected  during  the  acute  phase  and  during  the  symptomatic  phase  of  AIDS  only. Antibodies to HIV*1 and HIV*2 can be detected throughout virtually the total infection period, starting at or shortly after the acute phase and lasting till the end stage of AIDS. Therefore  the  use  of  highly  sensitive  antibody  assays  is  the  primary  approach  in serodiagnosis of HIV infection.

   The  wells  of  the  polystyrene  microplate  strips  are  coated  with  purified  recombinant gp**0, gp*1 (including group O) of HIV*1 and gp*6 of HIV*2 antigens. Human serum or plasma, diluted in sample diluent, are incubated in these coated wells. HIV specific antibodies,  if  present,  will  bind  to  the  solid  phase  HIV  antigens.  The  wells  are thoroughly  washed  to  remove  unbound  materials  and  HIV  antigens  labeled  with horseradish  peroxidase  is  added  to  the  wells.  These  labeled  antigens  will  bind  to  the antigen-antibody complexes previously formed and excess unbound labeled antigens are removed   by   washing.   Substrate   solution   containing   hydrogen   peroxide   and 3,3’,5,5’-tetramethylbenzidine(TMB)  is  then  added  to  each  well.  The  presence  of specific antibodies is indicated by the presence of a blue colour after substrate addition. Reaction  is  terminated  by  addition  of  sulphuric  acid.  The  intensity  of  the  colour  is measured  spectrophotometrically  at  **0nm  and  is  proportional  to  the  amount  of antibodies present in the specimen.

ELISA products
HAV IgM
HBsAg
Anti-HBs
Anti-HBc
HBeAg
Anti-HBe
PreS2Ag
HCV IgG
HDV Antigen
HDV IgG
HDV IgM
HEV IgG
HEV IgM
HGV IgG
Anti-HIV (type 1 and type 2)
EBV IgA
Anti-TP(syplilis)
AFP
G6PD
Rubella IgM
Rubella IgG
Toxoplasma IgM
Toxoplasma IgG
HSV (type 1 and type 2) IgM
HSV (type 1 and type 2) IgG
CMV