Human Anti-ganglioside antibody(GM3)ELISA Kit

タイトルまたは説明

Human Anti-ganglioside antibody(GM3)ELISA Kit
 

Please carefully read this instruction before using. This ELISA kit is based on the principle of double-antibody sandwich technique to detectHuman Anti-ganglioside antibody(GM3) Be used only for research purposes, not be used for medical diagnosis.       

Intended Use:
This kit is used to assay  the sample of Serum, blood plasma,Saliva, Urine, and other related tissue Liquid.

Test principle

The kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) to assay the level of Human Anti-ganglioside antibody(GM3)samples. AddHuman Anti-ganglioside antibody(GM3) monoclonal antibody Enzyme well which is pre-coated withHuman Anti-ganglioside antibody(GM3) monoclonal antibody, incubation; then, addHuman Anti-ganglioside antibody(GM3) antibodies labeled with biotin, and combined with Streptavidin-HRP to form immune complex; then carry out incubation and washing again to remove the uncombined enzyme. Then add Chromogen Solution A, B, the color of the liquid changes into the blue, And at the effect of acid, the color finally becomes yellow. The chroma of color and the concentration of the SubstanceHuman Anti-ganglioside antibody(GM3) sample were positively correlated.

 

Materials supplied in the Test Kit

 

Materials required but not supplied

1. *7 °C incubator     

2. Standard Enzyme reader

3. Precision pipettes and Disposable pipette tips     

4. Distilled water

5. Disposable tubes for sample dilution          

6. Absorbent paper

 

Summary Procedure

Preparing reagents, samples and standards

Add prepared samples and standards, antibodies labeled with enzyme, reacting 60 minutes at *7 °C

Plate washed five times, adding Chromogen solution A, B, reacting 10 minutes at *7 °C

Add stop solution

Measure the OD value within 10min

 

Package size:*6T per box.

Validity&Storage:Six months(**8°C)orTwelvemonths(**0°C)

[see label on the outer box for the specific date].