离岸价格
Get Latest Price300 ~ 500 /
|1 Box Minimum Order
国:
China
モデル番号:
SL2406Hu
离岸价格:
300 ~ 500 / Get Latest Price
ロケーション:
-
最低注文量の価格:
300
最低注文量:
1 Box
パッケージの詳細:
box package
納期:
3-4 days for ready goods
供給能力:
1000 Box per Week
支払いタイプ:
T/T, Western Union, Money Gram
製品グループ :
-
連絡先担当者 Echo
Hangzhou, Zhejiang
[Testing Process]
This kit employs Double Antibody Sandwich Technique. The principle of Double Antibody Sandwich is based on characteristics of the tested antigen with more than two valances which can identify coated antibody and detection antibody at same time. The specific process is as follows:
1. Connect the specific antibodies and solid phase carriers to form immobilized antibodies. Wash out uncombined antibodies and impurities. Seal the rest binding sites with irrelevant proteins.
2. Join under test with immobilized antibodies for contact reaction. After a while, combine antigens in and antibodies on carriers into the antigens complex. Wash out uncombined antibodies and impurities.
3. Add biotin labeling antibodies to combine with the antigens on immune complexes. Wash out the uncombined biotin labeling antibodies thoroughly. The enzyme amount on the carrier is now positively related to the amount of the tested substance in specimens.
4. Add horseradish peroxidase to label the avidins and incorporate them with the biotin labeling antibodies. Wash out the incorporated enzyme markers thoroughly. The enzyme amount on the carrier is now positively related to the amount of the tested substance in specimens.
5. Add substrates for coloring, and compute the concentration of specimens.
[Detection principle]
This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
[Intera assay Precision]≤ 8% [Inter assay Precision]≤ *2%
[Storage]-*0°C [Short-term should be placed 4°C(such as two weeks)]
[Validity]*2 months (**0°C).
[Reference]
1 .Seigel, L.J.et al.(***4) Science **3:**5.
2. Minami, Y.et al.(***3) Annu. Rev. Immunol. *1:**5.
Elisa kit packing method: all kits sent along with foam box,*8T/BOX or *6T/BOX
Shipping:By TNT/DHL/UPS/FEDEX(Door to Door) and it take **5 days to reach your hand.
国: | China |
モデル番号: | SL2406Hu |
离岸价格: | 300 ~ 500 / Get Latest Price |
ロケーション: | - |
最低注文量の価格: | 300 |
最低注文量: | 1 Box |
パッケージの詳細: | box package |
納期: | 3-4 days for ready goods |
供給能力: | 1000 Box per Week |
支払いタイプ: | T/T, Western Union, Money Gram |
製品グループ : | - |