Olaquindox metabolite (MQCA) ELISA kit

タイトルまたは説明

The enzyme converts the colorless chromogen into a blue product. The addition of the stop solution leads to a color change from blue  to  yellow.  The  color  intensity  is  measured  photometrically  at  **0  nm.  The  color development is inversely proportional to the MQCA concentration in the sample.
 During  the  incubation,  the  free  MQCA molecules  and  the  coupling  antigens compete for the anti-MQCA antibodies. Any unbound anti-MQCA antibodies and the complex of MQCA bound anti-MQCA antibodies are then removed in a washing step. Then, horseradish peroxidase labeled IgG are added to the microwells and bound by the bound anti-MQCA antibodies. After an incubation time of *0 min, the non-bound horseradish peroxidase labeled IgG are removed in a washing step. The bound enzyme activity is determined by the addition of substrate/chromigen .