2x Realab Green PCR Fast mixture Universal

离岸价格

Get Latest Price

- Minimum Order

国:

China

モデル番号:

离岸价格:

（交渉可能） Get Latest Price

ロケーション:

最低注文量の価格:

最低注文量:

パッケージの詳細:

納期:

供給能力:

支払いタイプ:

製品グループ :

Molecular Biology

今すぐお問い合わせください

Lablead biotech

China

200 信任点

1st 年

連絡先担当者 Ying

今すぐお問い合わせください

製品仕様

タイトルまたは説明

2x Realab Green PCR Fast mixture Universal

(Cat. No.: R***2)

Price:

$*8	$*0	$**5	$**2
1ml(**0T)	51ml(*0T)	01ml(***0T)	51ml(***0T)

Storage conditions: For long-term storage, please store at **0â„ƒaway from light. After the Mix is â€‹â€‹thawed, it can be stably stored at 4â„ƒaway from light for one month. Try to avoid repeated freezing and thawing.

Product Introduction

Taq SYBR® Green qPCR Premix is â€‹â€‹a qPCR reagent dedicated to the SYBR® Green I chimeric dye method. It is a 2× premix containing all qPCR components except primers and DNA samples, which can reduce the operation steps, shorten the sample addition time, and reduce the chance of contamination. Its core component is the antibody-modified hot-start Taq DNA polymerase, combined with a carefully optimized buffer system and PCR reaction promoter, so that the product has the characteristics of strong specificity and high amplification efficiency, effectively inhibits nonspecific amplification, and can accurately quantify templates in a wide concentration range to obtain stable and reliable qPCR results.

Instructions for use

1. Compatible models

Universal for all models

2. Precautions for use

â‘ Because the Mix is â€‹â€‹pre-mixed with dye, its storage or reaction system preparation process should be protected from strong light exposure;

â‘¡Before use, turn the Mix upside down and gently mix it. Do not vortex to mix it to avoid excessive bubbles.

3. Recommended qPCR reaction system


Reagent	amount of usage	final concentration
Taq SYBR® Green qPCR Premix	*0ul	1×
forward primer (*0uM)a	0.4ul	0.2uM
reverse primer(*0uM)a	0.4ul	0.2uM
DNAtemplateb	X ul	0~0 ng/0ul
Nuclea

a. The recommended final concentration of primers is 0.2uM. If the reaction effect is not good, it can be adjusted within the range of 0.1~1uM;

b. The recommended template addition amount is 1~2ul. If the template type is undiluted cDNA stock solution, the template addition amount should not exceed *0% of the total reaction system. Different types of DNA templates contain different numbers of target gene copies. If necessary, gradient dilution can be performed to determine the necessary amount of DNA template addition.

4. qPCR reaction program (can be adjusted appropriately according to the model)


Reagent	amount of usage	final concentration
Taq SYBR® Green qPCR Premix	*0ul	1×
forward primer(*0uM)a	0.4ul	0.2uM
reverse primer(*0uM)a	0.4ul	0.2uM
DNA Template b	X ul	0~0 ng/0ul
Nuclease-Free Water	To *0ul

国:	China
モデル番号:	-
离岸价格:	（交渉可能） Get Latest Price
ロケーション:	-
最低注文量の価格:	-
最低注文量:	-
パッケージの詳細:	-
納期:	-
供給能力:	-
支払いタイプ:	-
製品グループ :	Molecular Biology