Hydatid disease Antibody Rapid Test

Description

Summary The test take the Hydatid cysticercus antigen as the solid phase antigen to detect specific antibody against Hydatid in human serum. Reagents and contents 1) 8× Coated microtiter strips: ready to use 2) 1× Conjugate solution: ready to use 3) 1× Washing solution: ready to use 4) 1× Substrate A solution: ready to use 5) 1× Substrate B solution: ready to use 6) 1× Sample dilution: ready to use 7) 1× Stopping solution: ready to use 8) 1× Positive control: ready to use 9) 1× Negative control: ready to use *0) 1× Instruction sheet Test procedure 1 Adding samples and controls 2 Adding conjugate solution 3 Adding substrates Results 1 Observation with naked eyes before adding stopping solution Negative: No apparent blue appears in the sample wells. This indicates that no cysticercus antibody has been detected. Positive: Apparent blue appears in the sample wells. This indicates that the sample contains cysticercus antibody. Invalid: If no blue appears in the positive control well or apparent blue is observed in the negative control well, this indicates a possible error in performing the test. The test should be repeated using a new kit. 2 Judgment by microtiter plate reader Cut-off value = 2.1×A- (absorbance of negative control) Interpretation of results A**0 (patient) ≥ cut-off: anti- P.westermanii antibody positive A**0 (patient) ï&frac*4;œ cut-off: anti- P.westermanii antibody negative Notes 1 Store at **8, make the temperature of the kit return to room temperature before use. screw down the cap after use, don&#**9;t mix caps between diffirent bottles and don&#**9;t mix components between diffirent kits 2 Fill wells with distilled water when washing, *0seconds each time, pour out the liquid. prohibit to use tap water or any other water. 3 Recommand to judge results with instruments to improve comparability of testing. 4 It is best to incubate in water bath box if the instrument is available.