Tetracycline (TCs) ELISA kit

Description

[ Profile] Tetracycline(TCS) ELISA kit is used to detect in Tetracycline, oxytetracycline, chlortetracycline, doxycycline in honey, fish, shrimp , meat , tissue, is widely applied in honey, fish, shrimp , meat industry , ports and government, etc. This kit is Stable , Sample pretreatment and ELISA process is convenient, sensitivity is high , detect limit is 5 ppb. [Principle] This test kit is based on the competitive enzyme immunoassay for the detection of Tetracycline, oxytetracycline, chlortetracycline, doxycycline in the sample. The coupling antigens are pre-coated on the micro-well stripes. The TCs in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti- TCs antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for ***** (OD) value is determined in **0 nm, (OD) value of the sample has a negative correlation with TCs concentration in the sample the value is compared to the standard curve and the content of the corresponding TCs is subsequently obtained. [Technical data] Detection limit Tissue, milk, chicken, urine, feed ——————5ppb Liver, kidney—————————*0ppb Cross-reaction rate Tetracycline, ——————————**0% Oxytetracycline———————— *7.*9% Chlortetracycline ——————— *7.*3% Doxycycline ————————— *9.*9% Demecloycline———————— *3.*8% Metacycline————————— 5.*7% Recovery rate Tissue ————*0.5ï&frac*2;ž**3.8% Liver ———— *5.6ï&frac*2;ž**2.3% Kidney ——— *2.3ï&frac*2;ž**8.5% Honey ——— *1.7ï&frac*2;ž**5.6% Feed ——— *1.7ï&frac*2;ž**2.3% Milk ———— *8.5ï&frac*2;ž**1.5% Egg ———— *8.1ï&frac*2;ž**9.8%