Description

Description: The PCR Products/Agarose Gel DNA Purification Kit is designed for the rapid purification of PCR products, other enzymatic reaction product and for the efficient extraction of DNA fragments from agarose gel. The Kit is an innovative system that radically simplifies extraction and purification of nucleic acids from a variety of sources. Key to the system is the new silica matrix that specifically, but reversibly, binds DNA under certain optimal conditions, allowing proteins and other contaminants to be removed. Nucleic acids are easily eluted with Elution Buffer provided. Gel purification of DNA is a common technique for isolation of specific fragments from reaction mixtures. However, most methods either fail to completely remove agarose (which can lead to problems in downstream manipulations), shear the DNA, or result in very low yields. The Kit can recover DNA bands *0bp**0kb from all grades of agarose gel in yields exceeding *5%. The DNA band of interest is excised from the gel, dissolved in Binding Buffer, and applied to a DNA spin-column. Following a rapid wash step, DNA is eluted with Elution Buffer and is ready for other applications. The product is suitable for ligation, PCR, sequencing, restriction digestion, or various labeling reactions.