Sell Chicken growth hormone, GH ELISA KIT

Description

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING! Intended use This immunoassay kit allows for the in vitro quantitative determination of chicken growth hormone, GH concentrations in cell culture supernates, serum, plasma and other biological fluids. Introduction Growth hormone is a protein hormone of about **0 amino acids that is synthesized and secreted by cells called somatotrophs in the anterior pituitary. It is a major participant in control of several complex physiologic processes, including growth and metabolism. Growth hormone is also of considerable interest as a drug used in both humans and animals. Growth is a very complex process, and requires the coordinated action of several hormones. The major role of growth hormone in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-I. IGF-I stimulates proliferation of chondrocytes (cartilage cells), resulting in bone growth. Growth hormone does seem to have a direct effect on bone growth in stimulating differentiation of chondrocytes. IGF-I also appears to be the key player in muscle growth. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues. Test principle The microtiter plate provided in this kit has been pre-coated with an antibody specific to GH. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for GH and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then aTMB substrate solution is added to each well. Only those wells that contain GH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color.