Sell Human Pulmonary surfatcant-associated protein B, SP-B ELISA Kit

Description

Intended use This immunoassay kit allows for the in vitro quantitative determination of human SP-B concentrations in serum, plasma and other biological fluids. Introduction Pulmonary surfactant is primarily responsible for lowering the surface tension at the air-liquid interface in the alveoli, a process that is essential for normal respiration. Pulmonary surfactant is a mixture of phospholipids and proteins, including four distinct surfactant-associated proteins (SPs), SP-A, SP-B, SP-C, SP-D. SP-B(**9 kDa) and SP-C(4 kDa) are predominantly hydrophobic proteins that associate with lipids to promote the absorption of surfactant phospholipids and to reduce the surface tension in the alveoli. SP-A(****6 kDa) and SP-D (*3 kDa) are large multimeric proteins belonging to the family of calcium-dependent lectins, designated collectins, which contribute to the innate immune system. Both SP-A and SP-D have been shown to protect against microbial challenge through binding to the lipid components of the bacterial cell wall and facilitating the rapid removal of microbials. Test principle The microtiter plate provided in this kit has been pre-coated with an antibody specific to SP-B. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for SP-B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain SP-B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of **0 nm 2 nm. The concentration of SP-B in the samples is then determined by comparing the O.D. of the samples to the standard curve.