Sell Chicken Interferon gama , IFN-gama ELISA Kit

Description

Intended use This immunoassay kit allows for the specific measurement of total Chicken IFN-gamma concentrations in cell culture supernates, serum, and plasma. Introduction Interferon gamma (IFN- gama) is a multifunctional protein first observed as an antiviral activity in cultures of Sindbis virus-infected human leukocytes stimulated by PHA. Produced by T lymphocytes and natural killer (NK) cells, IFN-gama is now known to be both an inhibitor of viral replication and a regulator of numerous immunological functions. IFN-gama influences the class of antibody produced by B cells, upregulates class I and II MHC complex antigens and increases the efficiency of macrophage-mediated killing of intracellular parasites. Most of theactivities attributed to IFN-gama are believed to be mediated by IFN-gama-induced proteins. Theappearance of such proteins is a consequence of IFN-gama binding to a specific receptor that isdistinct from the receptor for IFN-gama Test principle This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IFN-gamma has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IFN-gamma present is bound by the immobilized antibody. An enzyme-linked polyclonal antibody specific for IFN-gamma is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IFN-gamma bound in the initial step. The color development is stopped and the intensity of the color is measured.