Sell Rabbit Vascuolar cell adhesion molecule 1, VCAM-1 ELISA kit

Description

Intended use This immunoassay kit allows for the specific measurement of rabbit VCAM*1 concentrations in cell culture supernates, serum, and plasma. Introduction VCAM*1 (CD**6), a member of the immunoglobulin superfamily, is a cell surface protein expressed by activated endothelial cells and certain leukocytes (such as macrophages). VCAM*1 expression is induced by IL*1β, IL*4, TNF-α and IFN-γ. VCAM*1 binds to leukocyte integrins VLA*4 and α4β7. The rat and mouse VCAM*1 proteins share approximately *6% amino acid similarity. During the inflammatory adhesion mechanism, activated integrins halt rolling leukocytes and attach them firmly to the vascular endothelium. They do this by binding to their ligands, for example VCAM*1, on endothelium. The VCAM*1: VLA*4/α4β7 interaction is also thought to be involved in the extravasation of white blood cells through the blood vessel wall to sites of inflammation. Test principle This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for VCAM*1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VCAM*1 present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for VCAM*1 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VCAM*1 bound in the initial step. The color development is stopped and the intensity of the color is measured. Human Vascuolar cell adhesion molecule 1, VCAM*1 ELISA kit Mouse Vascuolar cell adhesion molecule 1, VCAM*1 ELISA kit Porcine Vascuolar cell adhesion molecule 1, VCAM*1 ELISA Kit Rat Vascuolar cell adhesion molecule 1, VCAM*1 ELISA kit