Description

DNase I, RNase-free is an endonuclease that digests single- and double-stranded DNA. It hydrolyzes phosphodiester bonds producing mono- and oligodeoxyribonucleotides with *-phosphate and 3'-OH groups. The enzyme activity is strictly dependent on Ca2 and is activated by Mg2 and Mn2 ions: in the presence of Mg2, DNase I cleaves each strand of dsDNA independently, in a statistically random fashion , see picture below;   in the presence of Mn2, the enzyme cleaves both DNA strands at approximately the same site, producing DNA fragments with blunt-ends or with protruding termini of only one or two nucleotides in length.