Description
Test principle
The kit uses a double-antibody sandwich enzyme-linked immunosorbent
assay (ELISA) to assay the level of Rat
CASPASE3（CASPASE3） in samples. Add CASPASE3（CASPASE3） to
monoclonal antibody Enzyme well
which is pre-coated with Rat CASPASE3（CASPASE3）monoclonal
antibody, incubation; then, add CASPASE3
（CASPASE3）antibodies labeled with biotin, and combined with
Streptavidin-HRP to form immune complex;
then carry out incubation and washing again to remove the
uncombined enzyme. Then add Chromogen Solution
A, B, the color of the liquid changes into the blue, And at the
effect of acid, the color finally becomes yellow.
The chroma of color and the concentration of the Rat Substance
CASPASE3（CASPASE3） of sample were
positively correlated.
