Description
Source: Escherichia coli.
Molecular Weight: Protein A is a cell wall component produced
by several strains of Staphylococcus aureus that consists
of a single polypeptide chain and contains little or no
carbohydrate. Recombinant Protein A is
produced in E.Coli and functions essentially the same as
native Protein A. It consists of 5 IgGbinding domains E-D-A-B-C
aligned in series. It migrates with an apparent molecular mass of
*5kDa
in SDS-PAGE.
Quantity: 5mg/**0mg/1.0g
Purity: >*5% by SDS-PAGE analyses.
Specificity: The interaction between Protein A and IgG is not
equivalent for all species. Even within a species,
Protein A interacts with some subclasses of IgG and not
others. For instance, human IgG1, IgG2 and
IgG4 bind strongly, while IgG3 does not bind. There are also
many instances in which monoclonal
antibodies do not bind to Protein A, especially the majority
of rat immunoglobulins and mouse IgG1.
Applications: The Protein A molecule contains four
high-affinity (Ka =**8/mole) binding sites capable of
interacting with the Fc region from IgG of several species
including human and rabbit (Table 1).
Optimal binding occurs a pH 8.2, although binding is also good
at neutral or physiological conditions
(pH 7.**7.6).
Physical Appearance: Sterile Filtered White lyophilized
(freeze-dried) powder.
Formulation: Lyophilized with no additive.
Endotoxin: Less than 0.1EU/µg of Protein A as determined by
LAL method.
Reconstitution: Dissolve in distilled water or saline.
Storage: 2 years at **0°C. After reconstitution, aliquot and
store at **0°C. Avoid repeated freeze/thaw cycles.
Usage: This material is offered by Fast Innovation Bioscience
for research, laboratory or further
evaluation purposes. NOT FOR HUMAN USE
