Product name:Human Rotavirus,RV IgM ELISA Kit
Product description:This kit is a solid phase-phase sandwich enzyme
linked immuno sorbent assay (ELISA). Samples, including standards
of known target protein concentrations and unknowns are pipetted
into these wells. During the first incubation, the target protein
antigen and a biotinylated monoclonal antibody specific for target
protein are simultaneously incubated. After washing, the enzyme
(streptavidin-peroxydase) is added. After incubation and washing to
remove the entire unbound enzyme, a substrate solution which is
acting on the bound enzyme is added to induce a colored reaction
product. The intensity of this colored product is directly
proportional to the concentration of target protein present in the
samples.
