1. Principle This test kit is based on the competitive enzyme immunoassay for the detection of Rhodamine B in the Spice Oil,Oleoresins etc.The coupling antigens are pre-coated on the micro-well stripes. The Rhodamine in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Rhodamine antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with Rhodamine concentration in the sample. This value is compared to the standard curve and the content of the corresponding Rhodamine is subsequently obtained.
