タイトルまたは説明
intended use
This immunoassay kit allows for the in vitro quantitative
determination of Human
Sclerostin (SOST) concentrations in serum, Plasma, Urine,
tissue homogenates and Cell culture supernates and Other biological
fluids.
sample preparation
1. DL Sci&Tech Development Co.Ltd is only responsible for
the kit itself, but not for the samples consumed during the assay.
The user should calculate the possible amount of the samples used
in the whole test. Please reserve sufficient samples in
advance.
2. Please predict the concentration before assaying. If values
for these are not within the range of the standard curve, users
must determine the optimal sample dilutions for their particular
experiments.
3.If the samples are not indicated in the manual, a
preliminary experiment to determine the validity of the kit is
necessary.
4. Tissue or cell extraction samples prepared by chemical
lysis buffer may cause unexpected ELISA results due to the impacts
from certain chemicals.
5. Due to the possibility of mismatching between antigen from
other origin and antibody used in our kits (e.g., antibody targets
conformational epitope rather than linear epitope), some native or
recombinant proteins from other manufacturers may not be recognized
by our products.
6. Influenced by the factors including cell viability, cell
number or sampling time, samples from cell culture supernatant may
not be detected by the kit.
7. Fresh samples without long time storage is recommended for
the test. Otherwise, protein degradation and denaturalization may
occur in those samples and finally lead to wrong results.
calculation of the result
Average the duplicate readings for each standard, control, and
samples and subtract the average zero standard optical density.
Create a standard curve on log-log graph paper, with Human Sclerostin (SOST)
concentration on the y-axis and absorbance on the x-axis. Draw the
best fit straight line through the standard points and it can be
determined by regression analysis. Using some plot software, for
instance, curve expert 1.*0, is also recommended. If samples have
been diluted, the concentration read from the standard curve must
be multiplied by the dilution factor.
Note
The Stop Solution suggested for use with this kit is an acid
solution.Wear eye,hand,face and clothing protection when using this
material.
国: |
China |
モデル番号: |
-
|
离岸价格: |
600 ~ 700 / (交渉可能)
Get Latest Price
|
ロケーション: |
- |
最低注文量の価格: |
600 |
最低注文量: |
1 |
パッケージの詳細: |
ice bag |
納期: |
5-7 working days |
供給能力: |
- |
支払いタイプ: |
T/T |
製品グループ : |
ELISA Kit
|