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Introduction:
intended use
This immunoassay kit allows for the in vitro quantitative
determination of rat Insulin Like Growth Factor Binding Protein 2
(IGFBP2) concentrations in serum, Plasma, Urine, tissue homogenates
and Cell culture supernates and Other biological fluids.
sample preparation
1. DL Sci&Tech Development Co.Ltd is only responsible for
the kit itself, but not for the samples consumed during the assay.
The user should calculate the possible amount of the samples used
in the whole test. Please reserve sufficient samples in
advance.
2. Please predict the concentration before assaying. If values
for these are not within the range of the standard curve, users
must determine the optimal sample dilutions for their particular
experiments.
3.If the samples are not indicated in the manual, a
preliminary experiment to determine the validity of the kit is
necessary.
4. Tissue or cell extraction samples prepared by chemical
lysis buffer may cause unexpected ELISA results due to the impacts
from certain chemicals.
5. Due to the possibility of mismatching between antigen from
other origin and antibody used in our kits (e.g., antibody targets
conformational epitope rather than linear epitope), some native or
recombinant proteins from other manufacturers may not be recognized
by our products.
6. Influenced by the factors including cell viability, cell
number or sampling time, samples from cell culture supernatant may
not be detected by the kit.
7. Fresh samples without long time storage is recommended for
the test. Otherwise, protein degradation and denaturalization may
occur in those samples and finally lead to wrong results.
calculation of the result
Average the duplicate readings for each standard, control, and
samples and subtract the average zero standard optical density.
Create a standard curve on log-log graph paper, with rat Insulin
Like Growth Factor Binding Protein 2 (IGFBP2) concentration on the
y-axis and absorbance on the x-axis. Draw the best fit straight
line through the standard points and it can be determined by
regression analysis. Using some plot software, for instance, curve
expert 1.*0, is also recommended. If samples have been diluted, the
concentration read from the standard curve must be multiplied by
the dilution factor.
Note
The Stop Solution suggested for use with this kit is an acid
solution.Wear eye,hand,face and clothing protection when using this
material.
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