Mouse total cholesterol, TC ELISA Kit

タイトルまたは説明

Intended use This immunoassay kit allows for the use in vitro quantitative determination of mouse total cholesterol, TC concentrations in cell culture supernates, serum, plasma and other biological fluids. Test principle The microtiter plate provided in this kit has been pre-coated with an antibody specific to TC. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for TC and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain TC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of **0 nm ± 2 nm. The concentration of TC in the samples is then determined by comparing the O.D. of the samples to the standard curve.