Rat galanin, GAL ELISA Kit

タイトルまたは説明

Intended use This immunoassay kit allows for the specific measurement of rat galanin, GAL concentrations in cell culture supernates, serum, and plasma. Test principle This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for GAL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GAL present is bound by the immobilized antibody. An enzyme-linked polyclonal antibody specific for GAL is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GAL bound in the initial step. The color development is stopped and the intensity of the color is measured.